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Two new polyhydroxysteroids, 12β,16β,20-trihydroxycholesta-1,4-dien-3-one 16-acetate(1) and 24-methyl-12β, 16β,20-trihydroxycholesta-1,4-dien-3-one(2) were isolated from octocoral Sinularia sp. Their structures were elucidated primarily on the basis of 1D and 2D NMR and the mass spectroscopic studies. The cytotoxicity of these compounds against three tumor cell lines was also reported.