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目的：研究深度烫伤后局部组织中ＰＤＧＦｍＲＮＡ表达水平及其意义。方法：应用α－ ３２ＰｄＡＴＰ标记的ＰＤＧＦ－ ＡＡ和－ ＢＢ的ｃＤＮＡ 探针和斑点杂交测定大鼠深度烫伤后不同时相局部组织中ＰＤＧＦｍＲＮＡ的表达水平。结果：烫伤后组织中ＰＤＧＦ－ ＡＡ和－ ＢＢｍＲＮＡ的表达，较之对照组都有所升高，且在烫伤早期出现高峰，尤其是－ ＢＢｍＲＮＡ表达水平在深度烫伤后期又有持续升高，且绐终高于－ ＡＡｍＲＮＡ的表达水平。结论：ＰＤＧＦ在大鼠深度烫伤后的炎症反应和修复愈合过程中起着一定的作用。
Objective: To study the expression of PDGFmRNA in local tissues after deep scald and its significance. Methods: PDGF mRNA expression levels in local tissues at different phases after deep scald in rats were determined by cDNA probes of PDGF - AA and - BB labeled with α - 32PdATP and dot blot hybridization. Results: Compared with the control group, the expression of PDGF - AA and - BB mRNA in the scald tissue increased, and the peak appeared in the early stage of scald. In particular, the expression level of - BB mRNA increased continuously in the late stage of scald burn, Eventually higher than - AA mRNA expression levels. Conclusion: PDGF plays an important role in the inflammatory reaction and healing process after deep scald in rats.