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目的:探讨黄连对小鼠的抗焦虑作用及其机制。方法:将雄性小鼠随机分成5组(n=12),分别为模型对照组,阳性对照组,黄连水煎液高、中、低剂量组(1.8,0.9,0.45 g.kg-1),模型组ig生理盐水,阳性组ig地西泮2.5 mg.kg-1,连续ig给药5 d。末次给药0.5 h后,通过高架十字迷宫和明暗箱实验装置,观察黄连对小鼠焦虑的干预作用。用高效液相-荧光检测法测定脑内γ-氨基丁酸(GABA),谷氨酸(Glu),5-羟色胺的含量(5-HT)。结果:黄连水煎液能明显增加小鼠在高架十字迷宫装置上的开臂进入次数和开臂滞留时间,有增加小鼠明暗箱穿箱次数的趋势。不同组别小鼠脑内GABA的含量:黄连组>阳性组>模型组。结论:黄连水煎液有一定的抗焦虑活性。黄连抗焦虑机制与提高小鼠脑内GABA含量有关。
Objective: To investigate the anti-anxiety effect and mechanism of berberine in mice. Methods: Male mice were randomly divided into 5 groups (n = 12), model control group, positive control group, Coptis high, medium and low dose groups (1.8,0.9,0.45 g.kg-1) Model group ig saline, positive group ig diazepam 2.5 mg.kg-1, continuous ig administration for 5 d. 0.5 h after the last administration, through the elevated plus maze and light box experimental device to observe the intervention of Coptis on the anxiety of mice. The contents of GABA, Glu and 5-hydroxytryptamine (5-HT) in the brain were measured by HPLC-fluorescence method. Results: Coptis decoction could significantly increase the number of open arms and the open arms of the mice in the elevated plus maze device, which may increase the number of mice in the light box. The contents of GABA in different groups of mice: coptis group> positive group> model group. Conclusion: Coptis decoction has certain anti-anxiety activity. Coptis chinensis anxiolytic mechanism and increase brain GABA content in mice.