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确定幽门螺杆菌(Hp)感染的最简单方法是检测其相应抗体。酶联免疫吸附试验(ELISA)由于简单、快速,因此最为常用。但它存在交叉反应,且<10岁儿童的抗体效价显著低于其他病人。因此,必须对这种血清学方法进行改进。本项研究中比较ELISA和免疫印迹法在诊断儿童Hp感染中的差别。试验收集了80名有上消化道不适症状的儿童血清,分别用ELISA和Western印迹法(WB)检测相
The easiest way to determine H. pylori infection is to test its antibody. Enzyme-linked immunosorbent assay (ELISA) is the most commonly used because of its simplicity and speed. However, it is cross-reactive and antibodies in children <10 years of age are significantly lower than those in other patients. Therefore, this serological method must be improved. This study compared the ELISA and immunoblotting in the diagnosis of Hp infection in children the difference. Eighty sera from children with upper gastrointestinal symptoms were collected and tested by ELISA and Western blotting (WB)