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目的研究硫化氢(H2S)对七叶皂苷钠(SA)细胞毒性的保护作用。方法培养HK-2细胞分别建立H2S处理[培养基中添加H2S的供体硫氢化钠(NaHS)]和H2S关键合成酶胱硫醚β合成酶(CBS)、胱硫醚γ裂解酶(CSE)的抑制剂炔丙基甘氨酸(PPG)处理的实验模型。CCK-8实验检测细胞存活率;流式细胞凋亡实验检测细胞凋亡率。结果 SA处理HK-2细胞24h后,细胞存活率为(48.21±3.57)%,细胞凋亡率为(40.8±1.41)%。同时给予SA和NaHS处理,HK-2细胞存活率[(74.35±3.62)%]升高,凋亡率[(17.7±0.55)%]降低(P<0.01);相反,同时给予SA和PPG处理,对HK-2细胞的毒性作用增强,细胞存活率[(19.67±2.31)%]降低,细胞凋亡率[(76.91±2.36)%]升高(P<0.01)。结论 H2S能对抗SA对HK-2细胞的毒性作用,抑制细胞凋亡。
Objective To study the protective effect of hydrogen sulfide (H2S) on the cytotoxicity of sodium aescinate (SA). Methods H2S treatment [H2S donor sodium hydrosulfide (NaHS)] and H2S key enzyme cystathionine β synthase (CBS) and cystathionine γ lyase (CSE) were cultured in HK-2 cells. The experimental model of inhibitor propargylglycine (PPG) treatment. Cell viability was detected by CCK-8 assay; apoptosis rate was detected by flow cytometry. Results After treated with SA for 24 h, the cell survival rate was (48.21 ± 3.57)% and the apoptosis rate was (40.8 ± 1.41)%. At the same time, SA and NaHS treatment increased the survival rate of HK-2 cells (74.35 ± 3.62%) and decreased the apoptotic rate (17.7 ± 0.55%) (P <0.01) (19.67 ± 2.31)%], the apoptotic rate [(76.91 ± 2.36)%] increased (P <0.01), and the cytotoxicity to HK-2 cells was enhanced. Conclusion H2S can antagonize the cytotoxic effect of SA on HK-2 cells and inhibit the apoptosis of HK-2 cells.