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目的:探讨红参水提物对哮喘小鼠气道炎症的治疗作用及其机制。方法:把60只雌性BABL/c小鼠随机数字表法分为6组,每组10只,分别为正常对照组、哮喘模型组、红参水提物200 mg/kg剂量组、红参水提物100 mg/kg剂量组、红参水提物50mg/kg剂量组、地塞米松0.5 mg/kg组。在末次激发24 h后所有小鼠取左肺组织行HE染色,观察哮喘模型组肺组织炎症浸润情况,用酶联免疫吸附法(ELISA)观察小鼠支气管肺泡灌洗液(BALF)中细胞的总数和各种炎症细胞计数的变化以及白细胞介素-4(Interleukin 4,IL-4)、IL-5含量;取右肺组织用Western blot检测NF-κB p65蛋白的表达情况。结果:哮喘模型组小鼠与对照组相比较BALF中的炎症细胞计数、IL-4、IL-5水平增高,肺组织中的NF-κB p65表达水平显著高于对照组;红参水提物组有效剂量(100~200 mg/kg)和地塞米松组小鼠与哮喘组小鼠相比炎症细胞计数、IL-4、IL-5以及NF-κB p65蛋白表达水平均显著降低,具有显著的差异。结论:红参水提物在体内可能主要通过抑制NF-κB p65等核转录因子来调节Th2反应,以此来抑制气道炎症反应。
Objective: To investigate the therapeutic effect and mechanism of red ginseng aqueous extract on airway inflammation in asthmatic mice. Methods: Sixty female BABL / c mice were randomly divided into 6 groups (n = 10): normal control group, asthma model group, 200 mg / kg red ginseng water extract group, Extract 100 mg / kg dose group, red ginseng water extract 50 mg / kg dose group, dexamethasone 0.5 mg / kg group. The left lungs of all the mice were stained with HE at 24 h after the last challenge to observe the infiltration of inflammatory cells in the lung tissue of the asthmatic model group and the changes of the cells in the bronchoalveolar lavage fluid (BALF) by enzyme-linked immunosorbent assay (ELISA) The expression of NF-κB p65 protein in the right lung was detected by Western blot. Results: Compared with the control group, the number of inflammatory cells in BALF, the levels of IL-4 and IL-5 in BALF and the expression of NF-κB p65 in lung tissue of asthmatic model group were significantly higher than those in control group. Compared with the mice in the asthma group, the effective dose (100-200 mg / kg) and the dexamethasone group significantly reduced the inflammatory cell count, IL-4, IL-5 and NF-κB p65 protein expression The difference. Conclusion: The red ginseng aqueous extract may regulate the Th2 response mainly by inhibiting the nuclear transcription factor such as NF-κB p65 in vivo to suppress the airway inflammatory reaction.