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构建了一种基于AuNPs/p-ABA/MWCNTs-COOH修饰玻碳电极的电化学DNA传感器高灵敏度检测金黄色葡萄球菌的方法。根据金黄色葡萄球菌特有的nuc基因的序列编码,设计了特异性DNA探针,利用自组装法将其结合到基于纳米金修饰的电极上构成检测电极,运用DPV电化学检测法来检测电活性杂交指示剂阿霉素的信号。对金黄色葡萄球菌的nuc基因的特异性片段检测限可达5×10-13mol/L。此传感器的构建使传感技术在金黄色葡萄球菌的实时高效检测中有了初步的探索。此研究为传感技术在实时高效检测金黄色葡萄球菌中的应用奠定了基础。
A high sensitive detection method of Staphylococcus aureus based on electrochemical DNA sensor with AuNPs / p-ABA / MWCNTs-COOH modified glassy carbon electrode was constructed. According to the sequence encoding of Staphylococcus aureus specific nuc gene, a specific DNA probe was designed. The DNA probe was assembled on the electrode based on gold nanoparticles by self-assembly method. The DPV electrochemical detection method was used to detect the electrical activity Signal of hybridization indicator adriamycin. The specific detection limit of nuc gene of Staphylococcus aureus can reach 5 × 10-13mol / L. The construction of this sensor has enabled the initial exploration of sensing technology in real-time and efficient detection of Staphylococcus aureus. This study laid the foundation for the application of sensing technology in the real-time and efficient detection of Staphylococcus aureus.