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目的：探讨体内非靶向转染表达重组FN多肽CH50对肿瘤的抑制作用及其相关机制。方法：BALB/c小鼠接种肝癌细胞后,实验组采用基于流体动力学方法体内非靶向转染CH50真核表达质粒,对照组分别注射对照质粒或生理盐水。接种后观察肿瘤生长情况；RT-PCR检测治疗过程中肿瘤局部组织B7-1、B7-H1等基因的表达变化；流式细胞术检测分析肿瘤局部T淋巴细胞的数量。结果：体内非靶向基因转染表达CH50对肿瘤产生显著的抑制作用；肿瘤组织中B7-1、BT-H1等基因的表达随着肿瘤生长而上调；CH50治疗后可使B7-1/B7-H1及B7-1/B7-DC的比值显著增高,同时显著抑制IL-10和TGF-β基因的表达；CH50直接作用于瘤细胞可导致TGF-β基因表达下调；治疗组的肿瘤组织TIL数量显著高于对照组。结论：非靶向转染表达CH50可有效抑制肿瘤生长,对肿瘤微环境中免疫基因表达的调节是其作用机制的重要方面。
Objective: To investigate the inhibitory effect of non-targeted transfection of recombinant FN polypeptide CH50 on tumor growth in vivo and its related mechanisms. METHODS: BALB/c mice were inoculated with hepatoma cells. In the experimental group, CH50 eukaryotic expression plasmids were transfected in vivo using a non-targeted method based on fluid dynamics. The control group was injected with a control plasmid or physiological saline. The tumor growth was observed after inoculation; RT-PCR was used to detect the expression changes of B7-1, B7-H1 and other genes in the tumor tissue during the treatment; flow cytometry was used to analyze the number of local T lymphocytes. RESULTS: The expression of CH50 in non-targeted genes transfected in vivo had a significant inhibitory effect on tumors; the expression of B7-1, BT-H1 and other genes in tumor tissues was up-regulated with tumor growth; B7-1/B7 after CH50 treatment The ratios of -H1 and B7-1/B7-DC were significantly higher, and IL-10 and TGF-β gene expression were significantly inhibited; CH50 directly acting on tumor cells resulted in the down-regulation of TGF-β gene expression; tumor tissue TIL in treatment group The number was significantly higher than the control group. Conclusion: The non-targeted transfection of CH50 can effectively inhibit tumor growth. The regulation of immune gene expression in the tumor microenvironment is an important aspect of its mechanism of action.