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目的:探讨口腔黏膜癌前病变口腔白斑(OLK)、赤斑(EK)及鳞癌(OSCC)组织中染色体9p上D9S171、D9S1752、D9S1748、IFNA及3p上D3S1266、D3S643、D3S966的LOH、MSI及其与细胞增殖关系的研究。方法:应用聚合酶链反应-变性聚丙烯酰胺凝胶电泳-银染方法,检测OLK、EK及OSCC染色体9p上D9S171、D9S1752、D9S1748、IFNA及3P上D3S1266、D3S643、D3S966的微卫星位点的LOH及MSI,将检测结果与细胞增殖水平进行相关分析。结果:口腔癌前病变及鳞癌组织中9p、3p上7个位点均出现LOH和/或MSI。不同病理组别单个位点的LOH/MSI检出率无显著性差异(p>0.05)。但综合9p上4个位点或3p上3个位点或9p+3p上7个位点微卫星改变发生率,发现:9p,3p,9p+3p上LOH及LOH+MSI在不同病理组间差异显著。LOH,MSI改变状况与增殖水平关系密切。上皮异常增生程度加重,细胞增殖水平增高,LOH/MSI检出率增加。具体表现为9p、3p的LOH检出率与AgNOR计数呈正相关,MSI检出率与PCNA表达水平呈正相关。结论:9p和3p区域的基因异常是OSCC发生和发展过程高频分子事件,该区域可能存在抑癌基因。9p和3p上的微卫星改变状况在口腔癌的发生、发展中扮演重要角色,作用机制可能与其促进细胞增殖活性,使细胞无限增殖有关。
Objective: To investigate the LOH and MSI of D9S171, D9S1752, D9S1748, IFNA and D3S1266, D3S643 and D3S966 on chromosome 9p in oral leukoplakia (OLK), erythematous (EK) and squamous cell carcinoma (OSCC) Study on the Relationship with Cell Proliferation. Methods: The microsatellite loci of D3S1266, D3S643 and D3S966 on D9S171, D9S1752, D9S1748, IFNA and 3P on chromosome 9p of OLK, EK and OSCC were detected by polymerase chain reaction-denaturing polyacrylamide gel electrophoresis-silver staining LOH and MSI, the detection results and cell proliferation correlation analysis. Results: LOH and / or MSI occurred in 7 sites of 9p and 3p in oral precancerous lesions and squamous cell carcinoma. There was no significant difference in the detection rate of LOH / MSI between different pathological groups (p> 0.05). However, the incidence of microsatellite changes at 4 sites or 3p or 9p + 3p sites on 9p, 9p, 3p and 9p + 3p was found to be significantly different between different pathological groups Significant difference. LOH, MSI change status and proliferation are closely related. Increased degree of epithelial dysplasia, increased cell proliferation, LOH / MSI detection rate increased. Specifically, the detection rate of LOH with 9p and 3p was positively correlated with the AgNOR count, and the detection rate of MSI was positively correlated with the expression level of PCNA. Conclusion: The gene abnormalities in 9p and 3p regions are high frequency molecular events in the development and progression of OSCC. There may be tumor suppressor genes in this region. Microsatellite changes on 9p and 3p play an important role in the occurrence and development of oral cancer, which may be related to the promotion of cell proliferation and cell proliferation.