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在醋酸-醋酸盐缓冲液(pH=4)中,糖精和Nile蓝(尼罗蓝)反应生成一个能被甲基异丁酮(MIBK)萃取的离子缔合物,该缔合物在630nm处的摩尔吸光系数为5.8×10~4L mol~(-1)cm~(-1)该法选择性好。主要试剂 1.糖精贮备液:称取200 mg糖精钠盐用水稀释到200 ml,现用现稀至所需浓度。 2.醋酸-醋酸盐缓冲液(pH=4):90 ml 1M
In acetate-acetate buffer (pH = 4), saccharin reacts with Nile Blue (Nile Blue) to form an ion association compound that can be extracted with methyl isobutyl ketone (MIBK) at 630 nm The molar extinction coefficient of 5.8 × 10 ~ 4L mol ~ (-1) cm ~ (-1) has good selectivity. The main reagent 1. Saccharin stock solution: Weigh 200 mg of sodium saccharin diluted to 200 ml with water, are now available dilute to the desired concentration. Acetic acid-acetate buffer (pH = 4): 90 ml 1M