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目的 探讨胰岛淀粉样多肽免疫反应 (IAPP IR)细胞和 5 羟色胺 (5 HT)免疫反应细胞 (EC细胞 )在人胎小肠中的个体发生及IAPP与 5 HT的关系。 方法 免疫组织化学技术。 结果 胎期小肠内IAPP IR细胞仅见于十二指肠。 16周开始 ,在十二指肠绒毛上皮细胞间可见单个散在的IAPP IR细胞 ;2 2～ 2 7周 ,其细胞数量则逐渐增多 ,主要分布于肠腺中。EC细胞可见于胎期小肠各段 ,并随着胎龄增长 ,其细胞密度大小依次为十二指肠、空肠、回肠。 11周 ,在小肠 3段绒毛上皮和尚未分化完全的肠腺细胞间已可见该种细胞 ;17～ 2 1周 ,数量达最多 ,主要分布于绒毛根部和肠腺的上皮细胞间 ;2 2周后 ,EC细胞呈渐少趋势。免疫组织化学邻片单染比较 ,未见IAPP和 5 HT在同一细胞内有共存现象。 结论 IAPP、5 HT在人胎小肠的内分泌细胞中已有表达。IAPP IR细胞及EC细胞随着胎儿的发育而发生不同变化
Objective To investigate the association of IAPP IR and 5 HT immunoreactive cells (ECs) in human fetal small intestine and the relationship between IAPP and 5 HT. Methods Immunohistochemistry. Results IABP IR cells in the small intestine of the fetus were found only in the duodenum. From 16th week, single scattered IAPP IR cells were observed in the duodenal villous epithelial cells. The numbers of cells were gradually increased in 2 ~ 27 weeks and mainly distributed in the gut. EC cells can be seen in each stage of the small intestine, and with the growth of gestational age, the cell density followed by the duodenum, jejunum, ileum. 11 weeks, in the small intestine 3 villus epithelial and not yet fully differentiated intestinal gland cells have been seen between the cells; 17 ~ 21, the largest number, mainly distributed in the villus roots and intestinal gland epithelial cells; 2 2 weeks later , EC cells showed a decreasing trend. Immunohistochemical adjacent patch single dye comparison, no IAPP and 5 HT co-exist in the same cell. Conclusion IAPP and 5 HT have been expressed in endocrine cells of human fetal small intestine. IAPP IR cells and EC cells vary with the development of the fetus