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膨化倍数是爆裂玉米最重要的品质指标。以普通玉米自交系丹232和爆裂玉米自交系N04杂交构建的259个F2:3家系为定位群体,采用完全随机区组设计在郑州春播和夏播条件下测定了膨化倍数。利用覆盖玉米10条染色体的183对多态性分子标记构建连锁图,采用复合区间作图法(CIM)进行QTL定位分析,采用多区间作图法(MIM)分析定位QTL间的互作效应。共检测出22个QTLs,单个QTL的贡献率为3.07%～12.84%,累计贡献率为66.46%和51.90%。其中5个QTLs在两种环境条件下均检测到,3个QTLs(qPF-6-1、qPF-8-1和qPF-1-3)的贡献率大于10%。大多数QTLs的加性效应值大于显性效应,表现为加性、部分显性、显性和超显性基因作用方式的QTLs数目在两种环境下分别为4、5、0、2和2、5、2、2。仅6对(占2.60%)QTLs或标记区间存在显著互作效应,表现为AA、DA或DD互作方式。
Expanded multiples of burst corn is the most important quality indicators. A total of 259 F2: 3 pedigrees were constructed from a cross of common maize inbred line Dan 232 and popcorn inbred line N04, and the puffing multiples were measured in a completely randomized block design in spring and summer sowing in Zhengzhou. The linkage map was constructed by 183 pairs of polymorphic molecular markers covering 10 maize chromosomes. QTL mapping was performed using composite interval mapping (CIM), and the interaction between QTLs was analyzed using multi-interval mapping (MIM). A total of 22 QTLs were detected. The contribution rate of single QTL was 3.07% ~ 12.84%, the cumulative contribution rate was 66.46% and 51.90%. Five of the QTLs were detected under the two environmental conditions, and the contribution rate of three QTLs (qPF-6-1, qPF-8-1 and qPF-1-3) was more than 10%. Most of the QTLs had additive effects larger than the dominant ones. The number of QTLs that showed additive, partially dominant, dominant and over-dominant genes was 4, 5, 0, 2 and 2 , 5,2,2. Only six pairs (2.60%) of QTLs or marker intervals had significant interaction effects, showing the way of interaction of AA, DA or DD.