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目的探讨河南省人群中外源性化合物代谢酶CYP1A1、GSTM1、GSTT1、mEH和DNA修复酶XRCC1基因多态性与肺癌易感性的关系。方法采用病例-对照研究的方法,选取河南省209例肺癌患者为病例组,256例健康体检者为对照组,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测I相代谢酶基因CYP1A1,II相代谢酶基因GSTM1、GSTT1、mEH及DNA修复酶基因XRCC1的多态基因型。结果河南省人群中GSTM1缺失型,CYP1A1-exon7、mEH-exon3、XRCC1-194及XRCC1-280基因纯和突变型在病例组与对照组中的分布频率差异均有统计学意义(P<0.05),GSTM1基因缺失者与GSTM1基因阳性者相比发生肺癌的危险性升高(ORadj=1.76,95%CI:1.21-2.56,P=0.005);携带CYP1A1-exon7 Ile/val+val/val基因型的个体较携带CYP1A1-exon7 Ile/Ile基因型的个体发生肺癌的危险性升高(ORadj=1.65,95%CI:1.16-2.39,P=0.009);mEH-exon3突变基因型携带者与野生纯合型的个体相比发生肺癌的危险性升高(ORadj=1.77,95%CI:1.18-2.64,P=0.007);携带XRCC1-194 Arg/Trp+Trp/Trp基因型的个体较携带XRCC1-194 Arg/Arg基因型的个体发生肺癌的危险性升高(ORadj=1.55,95%CI:1.07-2.27,P=0.016);XRCC1-280 His/His基因型携带者较XRCC1-280 Arg/Arg+Arg/His基因型携带者发生肺癌的危险性升高(ORadj=2.21,95%CI:1.05-4.52,P=0.026)。CYP1A1-Msp1、GSTT1、mEH-exon4及XRCC1-399多态基因型在病例组与对照组中的分布频率差异均无统计学意义(P>0.05)。结论河南省人群中CYP1A1、GSTM1、GSTT1、mEH和XRCC1基因的分布与国内外相关报道有一定差异,其中CYP1A1-exon7、GSTM1、mEH-exon3、XRCC1-194及XRCC1-280基因位点的变异与河南省人群肺癌患癌危险度增高有关。
Objective To investigate the relationship between polymorphisms of exogenous compounds CYP1A1, GSTM1, GSTT1, mEH and DNA repair enzyme XRCC1 and susceptibility to lung cancer in Henan province. Methods A case-control study was conducted in 209 cases of lung cancer patients in Henan Province and 256 healthy controls as control group. PCR-RFLP was used to detect I The polymorphism genotypes of CYP1A1, phase II metabolizing enzyme genes GSTM1, GSTT1, mEH and DNA repair enzyme gene XRCC1. Results There was a significant difference in the frequency distribution of GSTM1 deletion, CYP1A1-exon7, mEH-exon3, XRCC1-194 and XRCC1-280 genes between the case group and the control group in Henan province (P0.05) (ORadj = 1.76, 95% CI: 1.21-2.56, P = 0.005). The CYP1A1-exon7 Ile / val + val / val genotypes were higher in those with GSTM1 gene deletion than those with GSTM1 gene (ORadj = 1.65, 95% CI: 1.16-2.39, P = 0.009) were higher in individuals with CYP1A1-exon7 Ile / Ile genotypes compared with those with genotypes of mEH-exon3 and wild-type (ORadj = 1.77, 95% CI: 1.18-2.64, P = 0.007); individuals with the XRCC1-194 Arg / Trp + Trp / Trp genotype had significantly higher risk of developing lung cancer than those with XRCC1- The 194 Arg / Arg genotype had an increased risk of developing lung cancer (ORadj = 1.55, 95% CI: 1.07-2.27, P = 0.016). Compared with XRCC1-280 Arg / Arg Patients with + Arg / His genotype had a higher risk of lung cancer (ORadj = 2.21, 95% CI: 1.05-4.52, P = 0.026). There were no significant differences in the frequency of distribution of CYP1A1-Msp1, GSTT1, mEH-exon4 and XRCC1-399 genotypes among cases and controls (P> 0.05). Conclusion The distributions of CYP1A1, GSTM1, GSTT1, mEH and XRCC1 genes in Henan population are different from those reported in China and abroad. The variation of CYP1A1-exon7, GSTM1, mEH-exon3, XRCC1-194 and XRCC1-280 loci Henan Province, the population of lung cancer risk increased.