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以椴树蜂花粉为原料,以甲醇作为提取剂对椴树蜂花粉进行提取,然后对DPPH清除率、·O-阴离子清除率、·OH-自由基清除率和还原力进行体外抗氧化能力测定,在DPPH清除率方面,甲醇组分DPPH清除能力最强,在浓度为5 mg/m L时,其清除能力接近100%;在·O-清除率方面,甲醇组分最强,在浓度为5 mg/m L时,清除率为80.03%;在·OH-自由基清除率方面,在浓度为5 mg/m L时,甲醇组分最强为29.14%;在还原力方面水组分更强。选取体外抗氧化效果较好的椴树蜂花粉甲醇提取物和最为常见的椴树蜂花粉水提取物进行体内抗氧化测定,分别测定昆明鼠血清中的SOD活性、MDA含量和T-AOC值,结果表明:椴树蜂花粉在体内和体外均有较好的抗氧化能力。
Using linden bee pollen as raw material and methanol as extracting agent, the pollen of linden tree was extracted, then the DPPH scavenging rate, · O-anion scavenging rate, · OH-radical scavenging rate and reducing power were measured in vitro. , The scavenging capacity of DPPH was the strongest in the scavenging of DPPH, and its scavenging capacity was close to 100% at the concentration of 5 mg / mL. The scavenging capacity of methanol was the strongest at the concentration of 5 mg / m L, the clearance rate was 80.03%. In the OH radical scavenging rate, the strongest methanol component was 29.14% at the concentration of 5 mg / m L, and the water component was more in terms of reducing power Strong. Antioxidant activity of linden bee pollen extract and the most common linden pollen extract were determined in vitro. SOD activity, MDA content and T-AOC value in Kunming mouse serum were determined. The results showed that linden bee pollen had good antioxidant capacity in vivo and in vitro.