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目的:探索戈那瑞林对大鼠初级感觉神经元膜GABA引起的去极化和GABA激活电流的调制作用。方法:应用细胞内记录和全细胞膜片钳技术分别在大鼠脊神经节(SG)标本和新鲜分离神经元进行实验。结果:GABA(10 μmol·L~(-1)—1mmol·L~(-1))在大多数神经元引起可为荷包牡丹碱(100μmol·L~(-1))所阻断的膜去极化。预加戈那瑞林(50μmol·L~(-1))可减少GABA引起的去极化,抑制率为79±22％(n=29),而戈那瑞林本身不产生膜反应或只引起轻微去极化。在11个细胞中有6个细胞GABA激活电流也为戈那瑞林的预处理所抑制,另5个细胞无改变或反应稍有增加。结论:戈那瑞林对初级感觉神经元GABA介导的去极化和GABA激活电流具有抑制作用。
Objective: To investigate the effect of gonadorelin on the modulation of GABA-induced depolarization and GABA-activated currents in rat primary sensory neurons. Methods: Spinal ganglion (SG) specimens and freshly isolated neurons were isolated from rat spinal cord by using in-cell recording and whole-cell patch-clamp technique. RESULTS: GABA (10 μmol·L -1 -1 mmol·L -1) caused membrane blockage by bicuculline (100 μmol·L -1) in most neurons polarization. Pregagarin (50 μmol·L -1) decreased GABA-induced depolarization with a mean inhibition rate of 79 ± 22% (n = 29), while gonadorelin did not produce membrane reaction or only Cause slight depolarization. GABA-activated currents in 6 of 11 cells were also inhibited by pretreatment of gonadorelin, with no change in the other 5 cells or a slight increase in response. CONCLUSION: Gonadorelin has an inhibitory effect on GABA-mediated depolarization and GABA-activated currents in primary sensory neurons.