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在NG108-15细胞株上,用荧光探针fura-2/AM测定细胞内游离钙([Ca~(2+)]1)浓度。结果表明:丁二酰cAMP(Bt_2cAMP)、腺苷酸环化酶激动剂forskolin和蛋白激酶C激动剂十四碳酰佛波醇酯(TPA)均能使([Ca~(2+)]i)升高。钙通道拮抗剂异搏定(verapamil)可以抑制forskolin、Bt_2cAMP和TPA引起的[Ca~(2+)]i增加。δ阿片受体激动剂[D-Pen~2,D-Pen~5)enkephalin(DPDPE)能抑制Bt_2cAMP和forskolin引起的[Ca~(2+)]i增加,但不影响TPA引起的[Ca~(2+)]i增加。提示DPDPE可抑制cAMP依赖的蛋白激酶引起的钙内流,而与蛋白激酶C(PKC)引起的钙内流无关。
Intracellular free calcium ([Ca ~ (2 +)] 1) concentration was determined on fluorescent probe fura-2 / AM on NG108-15 cell line. The results showed that both [Ca ~ (2 +)] i (Bt_2cAMP), adenosine cyclase agonist forskolin and protein kinase C agonist tetradecyl phorbol ester (TPA) ) Increased. Verapamil, a calcium channel antagonist, can inhibit the increase of [Ca ~ (2 +)] i induced by forskolin, Bt_2cAMP and TPA. δ-opioid receptor agonist (DPPDE) inhibited the increase of [Ca ~ (2 +)] i induced by Bt_2cAMP and forskolin but did not affect [Ca ~ (2 +)] i increase. Tip DPDPE can inhibit cAMP-dependent protein kinase-induced calcium influx, and protein kinase C (PKC) -induced calcium influx unrelated.