Objective:To explore the larvicidal and pupicidal activity of Euphorbia hirta(E.hirta) leaf extract and Bacillus sphaericus(B.sphaericus) against the malarial vector.Anopheles siephensi(An. stephensi).Methods:The larvicidal and pupicidal activity was assayed against An.stephensi at various concentrations ranging from(75-375 ppm) under the laboratory as well as field conditions. The LC_(50) and LC_(90) value of the E.hirta leaf extract was determined by probit analysis.Results: The plant extract showed larvicidal effects after 24 h of exposure;however,the highest larval mortality was found in the methanol extract of E.hirta against the first to fourth instars larvae and pupae of values LC_(50)=137.40,172.65,217.81,269.37 and 332.39 ppm;B.sphaericus against the first to fourth instars larvae and pupae of values LC_(50)= 44.29,55.83,68.51,82.19 and 95.55 ppm, respectively.Moreover,combined treatment of values of LC_(50)= 79.13,80.42,86.01,93.00 and 98.12 ppm,respectively.No mortality was observed in the control.Conclusions:These results suggest methanol leaf extracts of E.hirta and B.sphaericus have potential to be used as an ideal ecofriendly approach for the control of the malarial vector.An.stephensi as target species of vector control programs.This study provides the first report on the combined mosquito larvicidal and pupicidal activity of this plant crude extract and bacterial toxin against An.stephensi mosquitoes. Objective: To explore the larvicidal and pupicidal activity of Euphorbia hirta (E. hirta) leaf extract and Bacillus sphaericus (B.sphaericus) against the malarial vector. Anopheles siephensi (An. Stephensi). Methods: The larvicidal and pupicidal activity was assayed against The LC_ (50) and LC_ (90) value of the E. hirta leaf extract was determined by probit analysis. Results: The however, the highest larval mortality was found in the methanol extract of E. hirta against the first to fourth instars larvae and pupae of values ​​LC 50 (50) = 137.40, 172.65, 217.81, 269.37 and 332.39 ppm; B.sphaericus against the first to fourth instars larvae and pupae of values ​​LC 50 = 44.29, 55.83, 68.51, 82.19 and 95.55 ppm, respectively.Moreover, combined treatment of values ​​of LC 50 = 79.13, 80.42, 86.01, 93.00 and 98.12 ppm, respectively. No. was was was observed in the control .Conclusions: These results suggest methanol leaf extracts of E.hirta and B.sphaericus have potential to be used as an ideal ecofriendly approach for the control of the malarial vector. An.stephensi as target species of vector control programs. This study provides the first report on the combined mosquito larvicidal and pupicidal activity of this plant crude extract and bacterial toxin against An. Stephensi mosquitoes.