目的揭示丹酚酸B(Salvianolic Acid B,Sal B)对肝癌细胞株HepG2的杀伤作用。方法用不同浓度的丹酚酸B处理HepG2细胞,37℃培养24h。用RT-PCR检测促凋亡基因Bax的转录水平,并用流式细胞术检测细胞凋亡的水平。结果①100μmol/L、50μmol/L、25μmol/L等浓度的Sal B处理都能使HepG2细胞促凋亡基因bax的转录水平升高,其中100μmol/L处理组最为明显。②不同浓度的Sal B处理都能使HepG2细胞发生凋亡,其中100μmol/L处理组最为明显。结论 Sal B有促进HepG2细胞凋亡的作用。 Objective To reveal the killing effect of salvianolic acid B (Sal B) on HepG2 cells. Methods HepG2 cells were treated with different concentrations of salvianolic acid B and cultured at 37 ℃ for 24h. The transcription level of pro-apoptotic gene Bax was detected by RT-PCR, and the level of apoptosis was detected by flow cytometry. Results ① The same concentration of Sal B treatment of 100μmol / L, 50μmol / L, 25μmol / L could increase the transcription level of the pro-apoptotic gene bax in HepG2 cells, of which 100μmol / L treatment group was the most obvious. ② The apoptosis of HepG2 cells could be induced by different concentrations of Sal B, of which 100 μmol / L was the most obvious. Conclusion Sal B can promote the apoptosis of HepG2 cells.