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尽管吸烟人群中仅有部分患COPD,但吸烟确是导致COPD的主要因素。吸烟增加肺部易感性和引起小气道炎症。通过比较健康吸烟和不吸烟者支气管肺泡灌洗液(BALF)中肿瘤坏死因子(TNF)、自介素6和8(IL-6、IL-8)的浓度,以致肺泡巨噬细胞(AM)产生细胞因子的能力,观察吸烟对肺内细胞因子的影响。 方法和对象 无症状吸烟者9例(其中女5例,平均32.6±2.9岁)。平均吸烟史19.1±4.7年,无心肺疾病;不吸烟者9例(其中女3名,平均24.1±0.9岁)。两组肺功能均正常,6周内无上呼吸道感染和用药史。收集BALF分别计总细胞数和分类,分离AM并培养(普通培养或加入100ng/ml LPS培养),以及用生物学方
Although only a fraction of people smokers have COPD, smoking is a major cause of COPD. Smoking increases lung susceptibility and causes small airway inflammation. By comparing the concentrations of tumor necrosis factor (TNF), interleukin 6 and 8 (IL-6, IL-8) in bronchoalveolar lavage fluid (BALF) in both healthy and non-smokers such that alveolar macrophages (AM) The ability to produce cytokines, to observe the effects of smoking on the cytokines in the lungs. Methods and subjects 9 asymptomatic smokers (including 5 females, mean 32.6 ± 2.9 years). The average smoking history was 19.1 ± 4.7 years without heart and lung disease. There were 9 non-smokers (including 3 females, average 24.1 ± 0.9 years). Lung function was normal in both groups, with no history of upper respiratory tract infection and medication within 6 weeks. Total BALF was collected for total cell number and classification, AM was isolated and cultured (normal or 100 ng / ml LPS culture), and biopsies