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以实验室克隆的苏云金芽孢杆菌Vip3A-LS1基因为材料,利用原核表达载体pET-21b,构建重组载体表达蛋白,并测定了表达蛋白对美国白蛾和杨扇舟蛾幼虫的活性。结果表明:表达重组质粒pEV113经IPTG诱导得到了约88kD的Vip3A-LS1蛋白;该蛋白对美国白蛾和杨扇舟蛾均有一定的杀虫活性,80μg蛋白对初孵美国白蛾平均校正死亡率达到(53.02±0.43)%,体质量抑制率达到了80.60%,对杨扇舟蛾初孵幼虫平均校正死亡率达到(68.00±0.60)%,抑制作用均达到80%以上。
The Vibrio thuringiensis Vip3A-LS1 gene cloned from laboratory was used as material to construct recombinant vector pET-21b, and the activity of the expressed protein against larvae of American white moth and Helicoverpa armigera was determined. The results showed that the recombinant plasmid pEV113 induced Vip3A-LS1 about 88kD induced by IPTG. The protein had certain insecticidal activity against C. glaucescens and A. nymphaea. The average corrected mortality rate (53.02 ± 0.43)%, the body mass inhibition rate reached 80.60%. The average corrected mortality of newly hatched larvae of Helicoverpa armigera reached (68.00 ± 0.60)% and the inhibitory effect reached more than 80%.