论文部分内容阅读
目的探讨Apicidin对体外培养人舌鳞癌CAL-27细胞影响及作用机制。方法体外培养人舌鳞癌CAL-27细胞,采用不同浓度的Apicidin作为实验组,并设立对照组,倒置显微镜观察细胞形态,CCK-8法检测细胞增殖、TUNEL、流式细胞仪检测Apicidin对CAL-27细胞凋亡作用。结果 Apicidin可显著抑制CAL-27细胞的生长(P<0.05),呈时间剂量依赖性。通过TUNEL法、流式细胞仪检测显示CAL-27细胞的凋亡,并且使CAL-27细胞停留在G2期。结论 Apicidin能显著抑制CAL-27的体外生长并能诱导细胞凋亡。
Objective To investigate the effect and mechanism of Apicidin on cultured human tongue squamous cell carcinoma CAL-27 cells. Methods Human tongue squamous cell carcinoma CAL-27 cells were cultured in vitro. Different concentrations of Apicidin were used as the experimental group, and the control group was set up. The morphological changes of the cells were observed by inverted microscope. The cell proliferation was detected by CCK-8 assay, TUNEL and flow cytometry -27 cell apoptosis. Results Apicidin significantly inhibited the growth of CAL-27 cells (P <0.05) in a dose-and time-dependent manner. Flow cytometry revealed apoptosis of CAL-27 cells by TUNEL method and stayed CAL-27 cells in G2 phase. Conclusion Apicidin can significantly inhibit the growth of CAL-27 in vitro and induce apoptosis.