论文部分内容阅读
作者将12个常见致病的血清型的大肠杆菌与绿脓杆菌外毒素A载体蛋白结合制成多价菌苗,并在志愿者中进行安全性与免疫原性测定。 按制备大肠杆菌O18多糖-毒素A单价菌苗的方法制备多价O多糖结合菌苗。以酚-水法从O1、O2、O4、O6～O8、O12、O15、O18、O25及O75等12个血清型细菌的培养物中提取脂多糖,经1％乙酸处理后离心,上清过分子筛,经高碘酸钠氧化多糖,在有硼氢化钠存在情况下,将毒素A、乙二酸二酰肼和碳二亚胺的混合物与氧化的O多糖混合,过分子筛。将12种单价结合物加至瓶中冻干,终溶液中每种多糖含量为50μg/ml,多糖总量为600μg/ml,毒素A总量为800μg/ml。 作者用这种菌苗免疫3组共88名受试者,肌肉注射0.5ml菌苗（含300μg多糖）后连续7日测体温并记录临床症状,于接种后14、28、60、104、180天采集血标本作血清化学分析并用ELISA进行抗体应答测定。
The authors combined 12 common pathogenic serotypes of Escherichia coli with P. aeruginosa exotoxin A carrier protein into polyvalent vaccine and conducted safety and immunogenicity assays in volunteers. Multivalent O polysaccharide conjugate vaccine was prepared according to the method of preparing E. coli O18 polysaccharide-toxin A monovalent bacterin. Lipopolysaccharide was extracted from the culture of 12 serotypes of bacteria such as O1, O2, O4, O6 ~ O8, O12, O15, O18, O25 and O75 by phenol-water method, treated with 1% acetic acid, Molecular sieves, the polysaccharide is oxidized by sodium periodate, and the mixture of toxin A, oxalic acid dihydrazide and carbodiimide is mixed with the oxidized O polysaccharide in the presence of sodium borohydride to pass through the molecular sieve. Twelve kinds of monovalent conjugates were added to the bottle for lyophilization. The content of each polysaccharide in the final solution was 50 μg / ml, the total amount of polysaccharide was 600 μg / ml and the total amount of toxin A was 800 μg / ml. The authors used this vaccine to immunize three groups of 88 subjects, intramuscular injection of 0.5ml vaccine (containing 300μg of polysaccharides) after 7 consecutive days of body temperature and record the clinical symptoms, after inoculation 14,28,60,104,180 Blood samples were taken for serum chemical analysis and antibody response was measured by ELISA.