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激光照射对酶活性的影响主要取决于光源的波长和能量。系统研究了激光的波长和能量对酶活性的改变,并且对激光和发光二极管(LED)是否对酶活性改变具有相同的效果也进行了研究。分别采用能量为0~810J/cm2,激光波长分别为532、808、1064、1342nm,LED波长分别为640nm和810nm的激光照射磷脂酶C,通过高效薄层层析法(HPTLC)测量经过光源照射0.5、1、2、3、4、6、17、24h后神经酰胺的浓度来研究酶活性的改变。酶活性改变持续时间通过以上实验数据进行评估计算。在一定波长范围内激光和LED照射均可以提高磷脂酶C的活性,效果取决于光源的能量和波长,在经过照射后,酶活性的改变大约可以持续4h。研究结果表明在报道激光照射对酶活性改变的时候,酶活性改变持续时间应该作为一个主要的激光参数进行声明,还发现照射时选择波长、能量相同的激光源和LED,其对酶活性的改变具有同样的效果。
The effect of laser irradiation on enzyme activity depends mainly on the wavelength and energy of the light source. The wavelength and energy of the laser were systematically investigated for changes in enzyme activity and whether the laser and the light emitting diode (LED) had the same effect on the change of the enzyme activity was also studied. Phospholipase C was irradiated by laser with energy of 0 ~ 810J / cm2, laser wavelength of 532,808,1064,1342nm and LED wavelength of 640nm and 810nm, respectively. The content of phospholipase C was measured by high performance thin layer chromatography (HPTLC) 0.5, 1, 2, 3, 4, 6, 17, 24 h after the ceramide concentration to study changes in enzyme activity. The duration of the enzyme activity change is calculated by the above experimental data. In a certain wavelength range of laser and LED irradiation can increase the activity of phospholipase C, the effect depends on the energy and wavelength of the light source, after irradiation, the changes in enzyme activity can last about 4h. The results of the study indicate that the duration of change in enzyme activity should be stated as a major laser parameter when laser activity changes are reported and it is also found that laser sources and LEDs with the same wavelength and energy at irradiation change their enzymatic activity Have the same effect.