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Biosurfactant,produced by Pseudomonas aeruginosa BSZ-07,was added to the dee straw decomposing process to enhance the production of reducing sugars.Observed by Fourier Transform InfraRed(FT-IR)and Nuclear Magnetic Resonance(NMR)analysis, the purified biosurfactant was considered as a mixture of RL1 and RL2,which are two different types of rhamnolipids.Two different adding methods,adding the purified rhamnolipid and the on-site production of it were compared.The results showed that 0.5 g/L was the optimum concentration for adding purified rhamnolipid and the optimum temperature for on-site production was 30~C for the first 48 h and 34℃for the next 48 h.Under the optimum conditions,these two adding methods could improve the production of reducing sugar to 2.730 and 2.504 g/L,which was 22.30% and 12.20% higher than that of the rhamnolipid-free sample,respectively,which indicated that both of them were more effective than any other kind of surfactant discussed in this article.As the on-site production of rhamnolipid could omit the purification process,thus reducing the production cost effectively,it seemed to be a prospective adding method of the biosurfactant for enhancing rice straw decomposing.
Biosurfactant, produced by Pseudomonas aeruginosa BSZ-07, was added to the dee sediment decomposing process to enhance the production of reducing sugars. Observed by Fourier Transform InfraRed (FT-IR) and Nuclear Magnetic Resonance (NMR) analysis, the purified biosurfactant was considered as a mixture of RL1 and RL2, which are two different types of rhamnolipids. Two different adding methods, adding the purified rhamnolipid and the on-site production of it were compared. The results showed that 0.5 g / L was the optimum concentration for adding purified rhamnolipid and the optimum temperature for on-site production was 30 ~ C for the first 48 h and 34 ° C for the next 48 h.Under the optimum conditions, these two adding methods could improve the production of reducing sugar to 2.730 and 2.504 g / L, which was 22.30% and 12.20% higher than that of the rhamnolipid-free sample, respectively, which indicates that both both of them were more effective than any other kind of surfactant discussed in this article. As the on-s ite production of rhamnolipid could omit the purification process, thus reducing the production cost effectively, it seemed to be a prospective adding method of the biosurfactant for enhancing rice straw decomposing.