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考察了杜仲提取物中京尼平苷酸(1)、新绿原酸(2)、绿原酸(3)和隐绿原酸(4)在大鼠小肠的吸收动力学特性。采用大鼠在体肠循环灌流模型,建立了UPLC-MS/MS法测定1~4,考察了p H值、样品浓度、胆汁和P-糖蛋白(P-gp)抑制剂对1~4在体肠吸收的影响。结果显示,1在p H 7.4条件下吸收较差,2、4在不同p H值下的吸收有显著差异,而3对p H值不敏感。2、3在中浓度样品溶液(4.0 mg/ml)的3 h累积吸收率(A)显著高于高浓度样品(8.0 mg/ml),两者在高浓度下的吸收存在饱和现象。胆汁对1~4的吸收均有显著抑制作用。1~4在整个肠段均有吸收,主要吸收部位在小肠;P-gp抑制剂不影响杜仲提取物的肠吸收,提示1~4可能不是P-gp的底物。
The absorption kinetics of geniposidic acid (1), chlorogenic acid (2), chlorogenic acid (3) and cryptocerro acid (4) in Eucommia ulmoides extract were investigated in the rat small intestine. The rat model of intestinal perfused circulation was established by UPLC-MS / MS. The results of UPLC-MS / MS assay showed that p value, concentration of sample, inhibitor of bile and P-glycoprotein Intestinal absorption effects. The results showed that 1 was poorly absorbed at p H 7.4, 2 and 4 exhibited significant differences in absorption at different p H values, and 3 was not sensitive to p H values. The 3-h cumulative absorption (A) at 2,3 concentrations of medium sample solution (4.0 mg / ml) was significantly higher than that at high concentrations (8.0 mg / ml), both of which were saturated at high concentration. Bile on the absorption of 1-4 have a significant inhibitory effect. 1 to 4 are absorbed throughout the intestinal segment, the main absorption site in the small intestine; P-gp inhibitor does not affect intestinal absorption of Eucommia extract, suggesting that 1 to 4 may not P-gp substrate.