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本文研究了含编码乙型肝炎病毒表面抗原（HBsAg）基因的重组质粒DNA（pCMV－HBS）对HBsAg、抗·HBS免疫复合物（IC）诱生抗-HBs的影响。在Balb／c小鼠中，IC加PCMV－HBS（100μg）免疫组比单独使用IC或pCMV－HBsDNA组诱生的抗-HBs效价高，虽然略低于IC加氢氧化铝组，但无明显差异。在IC中加20μgpCMV-HBsDNA。经再次免疫亦可有效地诱生高效价的抗-HBs，可达PCMV－HBSAg100μg加IC免疫一次所诱生的杭体水平。然而在IC中加入1μgpCMV－HBs，即使再次免疫也不能使小鼠抗-HBS的阳转率达100％。此外在IC中加入载体质粒DNA（pCMV），亦可增强诱生抗-HBS。当DNA用量为100μg或20μg时，pCMV－HBs加入组诱生的抗-HBs效价略高于加入相同量的载体质粒DNA组。结果提示可用HBsAg-抗HBs免疫原性复合物加重组质粒DNA组建治疗性疫苗。
This article investigated the effect of recombinant plasmid DNA (pCMV-HBS) encoding the HBsAg gene on anti-HBs induced by HBsAg and anti-HBS immune complexes (ICs). In Balb / c mice, the ICV plus PCMV-HBS (100μg) immunized group had higher titers of anti-HBs induced than either IC or pCMV-HBsDNA alone, although slightly lower than that of the IC plus aluminum hydroxide group Significant differences. Add 20 μg of pCMV-HBsDNA to the IC. After re-immunization can effectively induce high-titer of anti-HBs, up to PCMV-HBSAg100μg plus IC immune once induced by the level of Hangzhou body. However, the addition of 1 μg pCMV-HBs to the IC did not result in a 100% positive rate of anti-HBS in mice even after re-immunization. In addition, the addition of vector plasmid DNA (pCMV) to IC also enhances the induction of anti-HBS. The anti-HBs titer induced by the addition of pCMV-HBs was slightly higher than the addition of the same amount of vector plasmid DNA DNA at 100 μg or 20 μg DNA. The results suggest that HBsAg-anti-HBs immunogenic complexes can be used to amplify plasmid DNA in the formation of therapeutic vaccines.