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目的 为了对SARS疑似患者进行快速早期诊断和进行血清学确认。方法 从SARS疑似患者含漱液与细胞病毒分离上清液中提取病毒RNA ,进行逆转录套式PCR反应 ,扩增SARS病毒特异性核酸片段 ,进行序列测定与序列比对。并对患者不同发病日期采集的血清标本进行SARS病毒ELISA抗体测定。结果 SARS患者含漱液标本和细胞病毒分离上清液中均能扩增出特异性核酸片段 ,经测序证实来自SARS冠状病毒。患者血清标本中SARS病毒抗体在发病后 18d开始呈阳性 ,以后随发病日期增加而升高。结论 逆转录套式PCR是一种早期、敏感、特异、快速检测传染性非典型肺炎疑似患者临床样本中SARS冠状病毒的理想方法。浙江省 3例SARS临床诊断病例的含漱液和血清标本经SARS病毒核酸和抗体检测 ,证实为SARS病例
Aim To provide rapid early diagnosis and serological confirmation of suspected SARS patients. Methods The virus RNA was extracted from the gargling and cytomegalovirus supernatant of patients with suspected SARS and reverse-transcribed nested polymerase chain reaction (PCR). SARS virus-specific nucleic acid fragments were amplified and sequenced and sequenced. Serum samples collected from patients with different onset dates were tested for SARS virus antibody. Results SARS patients gargle samples and cytomegalovirus isolated supernatants were able to amplify specific nucleic acid fragments confirmed by sequencing from SARS coronavirus. Serum samples of patients with SARS virus antibodies in the first 18 days after the onset of positive, with the date of onset increased. Conclusion RT-PCR is an ideal method for early, sensitive, specific and rapid detection of SARS coronavirus in clinical samples from suspected SARS patients. Gargle and serum samples of 3 cases of SARS diagnosed in Zhejiang Province were detected by SARS virus nucleic acid and antibody and confirmed as SARS cases