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目的:探讨毒物代谢酶GSTM1、GSTT1、GSTP1基因多态性与广东地区汉族人群喉癌遗传易感性的关系。方法:采用病例-对照研究的方法,运用PCR(多重PCR和ASA法)及PCR-RFLP、基因测序等技术检测233例广东地区汉族人群喉癌患者和102例健康对照组者外周血中GSTM1、GSTT1、GSTP1(Ⅰle/Ⅴal)基因的多态性。结合病历资料分析毒物代谢酶基因多态性与喉癌临床病理特征之间的关系。采用统计软件SPSS13.0处理数据,用Logistic回归模型进行因素间的相关检验,以OR值及其95%CI表示关联强度。结果:喉癌组GSTM1、GSTT1空白基因型频率显著高于对照组(OR=2.61、3.05,P<0.01)。重度吸烟与GSTT1空白基因型在喉癌致病中有协同作用(OR=3.51,95%CI为2.05～5.01;OR=2.99,95%CI为2.00～4.49)。有肿瘤家族史的患者GSTM1、GSTT1空白基因型比例较高。GSTM1、GSTT1空白基因型的患者晚期所占比例较大(P<0.05)。喉癌组与对照组中GSTP1多态基因频率差异无统计学意义。结论:GSTT1、GSTM1基因缺失与喉癌易感性关联,GSTT1空白基因型与吸烟特别是重度吸烟有协同作用而增加患癌易感性。GSTM1基因缺失致癌不与吸烟发生协同作用。GSTT1、GSTM1基因多态性可能影响喉癌的发展过程。GSTP1基因多态性可能与喉癌的遗传易感性无关。
Objective: To investigate the relationship between genetic polymorphisms of GSTM1, GSTT1 and GSTP1 and susceptibility to laryngeal cancer in Han nationality in Guangdong province. Methods: A case-control study was conducted to detect GSTM1 in peripheral blood of 233 laryngeal cancer patients and 102 healthy controls of Guangdong Han population by PCR (multiplex PCR and ASA), PCR-RFLP and gene sequencing. GSTT1, GSTP1 (Ile / Ⅴal) gene polymorphisms. The relationship between the gene polymorphism of toxic metabolizing enzymes and clinicopathological features of laryngeal carcinoma was analyzed based on the medical records. Statistical software SPSS13.0 was used to process the data, Logistic regression model was used to test the correlation between factors, and OR value and its 95% CI were used to express the correlation strength. Results: The frequencies of GSTM1 and GSTT1 in laryngeal carcinoma group were significantly higher than those in control group (OR = 2.61, 0.055, P <0.01). Severe smoking and GSTT1 null genotypes had synergistic effects in the pathogenesis of laryngeal cancer (OR = 3.51, 95% CI 2.05 ~ 5.01; OR = 2.99, 95% CI 2.00 ~ 4.49). Patients with a family history of cancer had a higher proportion of GSTM1 and GSTT1 null genotypes. Patients with GSTM1 and GSTT1 null genotypes had a greater proportion of patients with advanced disease (P <0.05). The frequency of GSTP1 polymorphism in laryngocarcinoma group and control group had no statistical significance. Conclusion: The deletion of GSTT1 and GSTM1 genes is associated with the susceptibility to laryngeal cancer. GSTT1 null genotypes have a synergistic effect with smoking, especially severe smoking, and increase the susceptibility to cancer. GSTM1 gene deletion carcinogen does not have synergy with smoking. GSTT1, GSTM1 gene polymorphism may affect the development of laryngeal cancer. GSTP1 gene polymorphism may not be related to genetic susceptibility to laryngeal cancer.