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目的采用膜杂交法对人乳头瘤病毒(HPV)进行基因分型,液基细胞学检测细胞分级,用作宫颈癌筛查手段的临床价值和意义。方法采用膜杂交法检测23个HPV基因型别(低危型:HPV6、11、42、43、45和高危型:HPV16、18、31、33、35、39、45、51、52、53、56、58、59、66、68、73、83、MM4)和液基细胞学(TCT)作为宫颈癌和癌前病变的筛查。结果781例临床样本中,HPV阳性360例;占46.02%,其中单一型HPV感染中,低危型占34%、高危型占60%、二型和二型以上混合感染占6%。TCT检查≥ASCUS 184例占27%。结论本地区宫颈疾患人群中,存在较高的HPV感染率;随着TCT级别的升高,HPV感染率、特别是高危型的感染率明显升高。HPV基因分型检测是有价值的辅助诊断技术,与细胞学联合,是最佳的宫颈癌筛查的方案。
Objective To investigate the clinical value and significance of human papillomavirus (HPV) genotyping by liquid membrane hybridization and cell sorting by liquid-based cytology. Methods Twenty-three HPV genotypes were detected by membrane hybridization (low-risk type: HPV6, 11, 42, 43, 45 and high-risk type: HPV16,18,31,33,35,39,45,51,52,53, 56, 58, 59, 66, 68, 73, 83, MM4) and liquid-based cytology (TCT) as a screening for cervical cancer and precancerous lesions. Results Of the 781 clinical samples, HPV was positive in 360 cases, accounting for 46.02%. Among them, 34% were low-risk type, 60% were high-risk type, and 6% were mixed type II and II. TCT examination ≥ASCUS 184 cases accounted for 27%. Conclusions There is a high prevalence of HPV infection in people with cervical diseases in this region. With the increase of TCT level, the infection rate of HPV, especially the high-risk type, has been significantly increased. HPV genotyping is a valuable adjunctive diagnostic technique that, in conjunction with cytology, is the best protocol for cervical cancer screening.