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溃疡性结肠炎急性加剧时,结肠组织损害可能由浸润的嗜中性白细胞(PMN)介导。但循环的PMN(浸润性PMN的来源)似乎正常,因为溃疡性结肠炎病人并无全身性炎症,而且体外PMN对各种物质的刺激如细菌肽甲酰基-甲硫氨基酸-亮氨酰-腩氨酸(FMLP)或佛波醇酯(PMA)的反应正常,换句话说,途径和/或浸润性PMN接触结肠腔内容物时可引发或加剧PMN功能,特别是合成氧自由基(OFR)的能力。为验证这假设,作者评估了9例对照病人或9例“活动性”溃结病人的PMN在预先接触直肠透析液后反应性产生OFR的能力。透析袋内含右旋糖酐和12kDa截止点。通过Luminol增强化学发光来检测PMN悬液产生的OFR量。每天取自1/10正常人的新鲜PMN(0.5～2×10~6细胞/ml)加入1ml的PBS缓冲液中。在每一试管用浓度为100nM或1nM的FML
When ulcerative colitis is acutely aggravated, colon tissue damage may be mediated by infiltrating neutrophils (PMNs). However, circulating PMN (source of invasive PMN) appears to be normal because there is no systemic inflammation in patients with ulcerative colitis, and in vitro PMN stimulation of various substances such as the bacterial peptide formyl-methionine-leucyl- (FMLP) or phorbol ester (PMA), in other words, the PMN function can be elicited or exacerbated by the pathways and / or invasive PMNs in contact with the luminal contents of the colon, in particular the synthesis of oxygen free radicals (OFRs) Ability. To test this hypothesis, the authors assessed the PMN’s ability to reactively produce OFR after pre-exposure to rectal dialysate in nine control patients or nine “active” patients with UC. The dialysis bag contains dextran and a 12 kDa cut-off point. The amount of OFR produced by PMN suspensions was measured by Luminol enhanced chemiluminescence. Fresh PMN (0.5 ~ 2 × 10 ~ 6 cells / ml) taken from 1/10 normal human beings were added to 1ml PBS solution daily. FML at a concentration of 100 nM or 1 nM in each tube