论文部分内容阅读
目的通过建立小鼠心梗模型,探讨应用超高分辨率超声系统评价心梗小鼠心脏结构及心功能方面的应用价值。方法 25只雄性C57BL/6小鼠随机分为2组,即开胸结扎左冠状动脉前降支建立心梗模型组(n=15)和仅在相同结扎位置穿针,不结扎的方法建立假手术组(n=10)。术前3 d和术后1周采用二维、M型、脉冲多普勒(PWD)、组织多普勒(TDI)和EKVTM超声技术对心梗小鼠心脏形态结构、室壁运动以及心功能进行评价。术后1周,取小鼠心脏进行HE染色并观察心肌组织结构。结果二维超声与HE色染均显示,心梗模型组心肌有明显的梗死区;心梗模型组与假手术组比较,心室明显扩大,室壁明显变薄,心室收缩功能与舒张功能均显著下降(P<0.05);局部室壁运动受阻严重,心室发生重构。结论采用2D超声心动图联合M型、PWD、TDI以及EKVTM超声心动图观察小鼠心梗模型能够更精准敏感地判断心肌梗死位置和梗死程度,为临床心肌梗死诊断、治疗和预后评估提供了参考价值。
Objective To establish a myocardial infarction model in mice to evaluate the value of ultra-high resolution ultrasound system in the evaluation of cardiac structure and cardiac function in myocardial infarction mice. Methods Twenty-five male C57BL / 6 mice were randomly divided into two groups: the MI group (n = 15) undergoing thoracotomy with ligation of the left anterior descending coronary artery and the establishment of a fake Surgery group (n = 10). Three days before operation and one week after operation, cardiac morphology, wall motion and cardiac function were evaluated by two-dimensional, M-mode, pulsed Doppler (PWD), Tissue Doppler (TDI) and EKVTM ultrasonography Evaluation. One week after operation, the hearts of mice were taken for HE staining and myocardial structure was observed. Results Two-dimensional ultrasonography and HE staining showed that myocardial infarction model group myocardial infarction area; myocardial infarction model group and sham operation group, ventricular enlargement significantly, ventricular wall thinning, ventricular systolic function and diastolic function were significant (P <0.05). Local wall motion was blocked severely and ventricular remodeling occurred. Conclusion 2D myocardial echocardiography combined with M type, PWD, TDI and EKVTM echocardiography can be used to determine myocardial infarction location and infarction more accurately and accurately, which provides reference for the diagnosis, treatment and prognosis of clinical myocardial infarction. value.