新生儿肺透明膜病是严重危及新生儿生命的疾病之一。目前,关于实验性肺透明膜病的模型国内尚无报道。水文通过大白鼠实验性肺透明膜病的建立,探讨其发生与病变特征。材料与方法实验动物为SD大白鼠,共48只。其中实验组(气管内注入透明膜液与吸入Ccl_4,3小时组10只、18小时组11只、24小时组11只)共32只。对照组A(单纯吸入Ccl_4)8只。对照组B(健康动物)8只。体重为150～200g/只。雌雄兼有,实验组在气管内注入透明膜液前预吸入低剂量(300mg/m~3)的Ccl_4,每次15分钟,共计12天。实验组大白鼠应用非暴露式气管注入法将肺透明膜液(每100ml中组成:同种血浆80ml、人胎羊水16ml、人血浆4ml、青霉素钠盐10万U)以1.2ml/只迅速均匀地注入经乙醚轻度麻醉的大白鼠双侧肺内。分别 Hyaline membrane disease of newborns is one of the diseases that seriously endanger the life of the newborn. At present, there is no report on the experimental model of hyaline membrane disease in China. Hydrology through the establishment of experimental rat hyaline membrane disease, explore the occurrence and lesion characteristics. Materials and methods Experimental animals SD rats, a total of 48. The experimental group (intratracheal instillation of transparent membrane fluid and inhaled Ccl_4,3 hours group of 10, 18 hours group of 11, 24 hours group of 11) a total of 32. Control group A (inhaled Ccl_4 only) 8. Control group B (healthy animals) 8. Weight is 150 ~ 200g / only. Both male and female, the experimental group pretreatment of low dose (300mg / m ~ 3) of Ccl_4, 15 minutes each for a total of 12 days before intratracheal injection of clear membrane fluid. The experimental group of rats using non-exposed tracheal injection of lung transparent membrane fluid (composed of 100ml per 100ml of the same plasma 80ml, human fetal amniotic fluid 16ml, human plasma 4ml, penicillin sodium 100000 U) to 1.2ml / only rapid uniform Into the ether slightly anesthetized rats bilateral lungs. respectively