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目的:研究自体骨髓间质干细胞移植对大鼠缺血性脑损伤的治疗作用及机制。方法:采用线拴法制作成年雄性SD大鼠脑缺血再灌注模型40只,随机分成磷酸盐缓冲液对照组和骨髓间质干细胞移植治疗组各20只,分别在1周后经颈动脉将标记5-溴脱氧尿嘧啶的2×106骨髓间质干细胞悬液和等体积磷酸盐缓冲液植入脑缺血大鼠体内。术后每天进行神经功能缺损评分,再灌注后1,2,3,4周分别取受损伤脑组织,检测不同时间点体感诱发电位与组织病理学,并采用免疫组化方法检测脑源性神经营养因子的表达。结果:治疗组在移植后各时间点神经功能缺损评分均明显低于对照组(P<0.05);治疗组神经细胞变性坏死数量减少,水肿明显减轻,体感诱发电位在各时间点的恢复较对照组差异均有统计学意义(P<0.05);治疗组脑源性神经营养因子表达水平明显高于对照组(P<0.05)。结论:自体骨髓间质干细胞移植对大鼠缺血性脑损伤有治疗作用,其机制可能与分泌脑源性神经营养因子等神经保护性因子有关。
Objective: To study the therapeutic effect and mechanism of autologous bone marrow mesenchymal stem cell transplantation on ischemic brain injury in rats. Methods: Forty adult male Sprague-Dawley (SD) rats with cerebral ischemia-reperfusion injury were randomly divided into phosphate buffer control group and bone marrow mesenchymal stem cell transplantation group, 20 rats in each group were treated with carotid artery 2 × 106 mesenchymal stem cells labeled with 5-bromodeoxyuridine and an equal volume of phosphate buffer were implanted into the cerebral ischemic rats. Neurological deficit scores were taken every day after operation. Injured brain tissues were harvested at 1, 2, 3 and 4 weeks after reperfusion. Somatosensory evoked potentials and histopathology were detected at different time points. Brain-derived nerve Trophic factor expression. Results: The scores of neurological deficit in the treatment group at each time point after transplantation were significantly lower than those in the control group (P <0.05). The number of the degenerative and necrotic neurons in the treatment group was decreased and the edema was obviously alleviated. The recovery of somatosensory evoked potentials (P <0.05). The expression of brain-derived neurotrophic factor in the treatment group was significantly higher than that in the control group (P <0.05). CONCLUSION: Autologous bone marrow mesenchymal stem cell transplantation has a therapeutic effect on ischemic brain injury in rats. The mechanism may be related to the secretion of neuroprotective factors such as brain-derived neurotrophic factor.