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小麦赤霉病的致病菌禾谷镰刀菌(Fusarium graminearum Schw.)能产生多种有致病活性的代谢物。其中,脱氧雪腐镰刀菌烯醇(DON)系引起赤霉病的主要毒素。本项目在研究DON对小麦的病理生理作用的基础上建立DON应用于抗赤霉病细胞工程的方法程序,筛选出抗赤性丰产性兼优的细胞无性系。主要结果如下: 一、发现DON的类生长激素活性:DON在小麦组织培养过程不但拮抗2,4—D的效应,而且对愈伤组织的分化和再生植株的生长表现低浓度促进,高浓度抑制的二重作用。DON对小麦黄化胚芽鞘的伸长生长,叶肉
Fusarium graminearum Schw., A pathogen of wheat scab, produces a variety of pathogenic activity metabolites. Among them, deoxynivalenol (DON) is the major toxin responsible for scab. Based on the study of the pathophysiological effects of DON on wheat, this project established a method for DON application in the cell engineering of scab resistance and screened the cell clones with high yield and good resistance to red taint. The main results are as follows: First, we found that DON growth hormone activity: DON in wheat tissue culture process not only antagonizes the effect of 2,4-D, but also on the differentiation of callus and the growth of regenerated plants showed low concentration, high concentration inhibition The dual role. DON growth of wheat yellow coleoptile sheath, mesophyll