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目的探讨对瞬时受体电位香草酸受体1(TRPV1)及转化生长因子β2(TGF-β2)的检测在儿童UACS中的诊断价值。方法选择32例于我院行腺样体手术的腺样体肥大患儿,其中上气道咳嗽综合征(UACS)组16例,对照组16例。所有患儿均于术前采血进行ELISA检测,术中取腺样体标本进行免疫组化试验。结果 UACS组与对照组相比,用ELISA法和免疫组化法进行检测,TRPV1及TGF-β2的检测值为(14.84±4.38)pg/ml和(159.02±2.17)pg/ml,较对照组[(4.90±5.64)pg/ml和(101.49±1.70)pg/ml]明显升高,差异有统计学意义(P<0.05)。ELISA与免疫组化检测结果之间无统计学意义的相关性。免疫组化的敏感性较高,阴性预测值较高,ELISA的特异性较高。结论 TRPV1及TGF-β2的ELISA及免疫组化检测对UACS有辅助诊断价值。此两项诊断试验具有一定程度的互补性。
Objective To investigate the diagnostic value of transient receptor potential vanilloid receptor 1 (TRPV1) and transforming growth factor β2 (TGF-β2) in children with UACS. Methods Thirty-two children with adenoid hypertrophy undergoing adenoid surgery in our hospital were enrolled. Among them, 16 were in the upper airway cough syndrome (UACS) group and 16 in the control group. All children were preoperative blood for ELISA testing, intraoperative adenoid specimens for immunohistochemistry. Results The serum levels of TRPV1 and TGF-β2 in UACS group were (14.84 ± 4.38) pg / ml and (159.02 ± 2.17) pg / ml, respectively, compared with the control group [(4.90 ± 5.64) pg / ml and (101.49 ± 1.70) pg / ml] were significantly increased, the difference was statistically significant (P <0.05). There was no statistically significant correlation between ELISA and immunohistochemical results. Immunohistochemical high sensitivity, high negative predictive value, ELISA specificity. Conclusion The ELISA and immunohistochemical detection of TRPV1 and TGF-β2 have diagnostic value for UACS. The two diagnostic tests have some degree of complementarity.