论文部分内容阅读
为探索分离培养人半月板纤维软骨细胞的简便实用方法 ,采用胰蛋白酶和胶原酶联合消化的方法 ,简便快速地获得大量成活率高的人半月板纤维软骨细胞。在F 12培养液中进行原代和传代培养 ,并对传代培养纤维软骨细胞进行了免疫组化鉴定 ,光镜及超微结构的观察。结果显示 ,光镜下 ,原代培养细胞呈线形 ,单层排列 ,电镜可见细胞内有丰富的粗面内质网及线粒体 ,细胞呈多极性 ,表面有突起 ;纤维软骨细胞免疫组化Ⅱ型胶原染色阳性。提示本实验建立的人纤维软骨细胞分离培养方法是一种可行的方法
In order to explore a simple and practical method to separate and culture human meniscal fibrochondrocytes, a large number of human meniscal fibrochondrocytes with high survival rate were obtained easily and rapidly by trypsin and collagenase digestion. Primary culture and subculture were carried out in F12 culture medium. The subcultured fibrochondrocytes were identified by immunohistochemistry, light microscopy and ultrastructure. The results showed that under the light microscope, the primary cultured cells were linear and arranged in a single layer. Electron microscopy showed abundant rough endoplasmic reticulum and mitochondria inside the cells. The cells were multipolar with protrusions on the surface. The immunohistochemistry of fibrochondrocytes Ⅱ Type collagen staining positive. It is suggested that the method of human chondrocyte isolation and culture established in this experiment is a feasible method