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目的探讨一氧化氮释放型阿司匹林(NO-ASA)对小鼠皮下种植前列腺癌的生长及肿瘤血管形成的抑制作用。方法皮下荷前列腺癌小鼠体内注射NO-ASA后监测肿瘤生长情况;处死小鼠后以ELISA法检测小鼠血清VEGF的表达,实时定量PCR法检测肿瘤中VEGF mRNA的表达,免疫组化法检测肿瘤中CD34的表达并量化微血管数目,以未注射NO-ASA的皮下荷前列腺癌小鼠为对照。受精鸡胚尿囊膜(HET-CAM)试验检测NO-ASA在体外对血管形成的影响。结果小鼠体内注射NO-ASA后皮下种植的前列腺癌的生长受到抑制,小鼠血清以及肿瘤组织的VEGF表达明显降低,肿瘤组织中的CD34表达减少,与对照组相比差异有统计学意义(P<0.05,P<0.01)。NO-ASA对受精鸡卵尿囊膜上的血管生长也有明显抑制作用。结论 NO-ASA可抑制前列腺癌肿瘤生长和血管形成。
Objective To investigate the inhibitory effect of nitric oxide-releasing aspirin (NO-ASA) on the growth and tumor angiogenesis of prostate cancer subcutaneously in mice. Methods Mice were subcutaneously injected with NO-ASA to monitor their tumor growth. The mice were sacrificed and the serum VEGF expression was detected by ELISA. The expression of VEGF mRNA was detected by real-time quantitative PCR. The expression of VEGF mRNA was detected by immunohistochemistry The expression of CD34 in the tumor and the quantification of the number of microvessels were compared with subcutaneous-loaded prostate cancer mice not injected with NO-ASA. Fertilized chick embryo allantoic membrane (HET-CAM) assay was used to examine the effect of NO-ASA on angiogenesis in vitro. Results The growth of subcutaneously implanted prostate cancer was inhibited after NO-ASA was injected in vivo. The expression of VEGF in serum and tumor tissue of mice was significantly decreased, while the expression of CD34 in tumor tissue was decreased. The difference was statistically significant compared with control group P <0.05, P <0.01). NO-ASA also significantly inhibited the growth of blood vessels on the membrane of fertilized chicken’s egg allantoic membrane. Conclusion NO-ASA can inhibit tumor growth and angiogenesis in prostate cancer.