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目的 探讨乙型肝炎病毒 (HBV)对肾脏的致病作用。方法 应用地高辛素标记的HBVDNAx+c段探针原位杂交 ,检测13例HBV相关性肾炎患者肾活检组织石蜡包埋切片HBVDNA的存在状况。结果 HBVDNA阳性7例 (53.8% )。阳性信号主要见于肾小管上皮细胞和肾小球系膜细胞 ,其次是肾小球上皮细胞。呈核型或浆核型。其病理类型均为膜性肾病。结论 HBV相关性膜性肾病患者肾组织内存在HBVDNA ,肾组织内存在的HBV可能直接导致肾脏损伤 ,或通过原位免疫复合物形成致病
Objective To investigate the pathogenic effect of hepatitis B virus (HBV) on the kidney. Methods The presence of HBVDNA in paraffin-embedded sections of renal biopsies of 13 patients with HBV-associated glomerulonephritis was detected by in situ hybridization with digoxigenin-labeled HBVDNAx + c probe. Results HBVDNA positive in 7 cases (53.8%). Positive signals mainly found in renal tubular epithelial cells and mesangial cells, followed by glomerular epithelial cells. Karyotype or plasma-nuclear type. The pathological types are membranous nephropathy. Conclusions HBVDNA is present in the kidney of patients with HBV-associated membranous nephropathy. The presence of HBV in the kidney tissue may directly lead to kidney damage or pathogenicity through in situ immune complexes