目的研究氟化钠亚慢性染毒对雌性小鼠所产生的氧化损伤。方法取雌性小鼠40只,随机分成4组,每组10只。即对照组,氟低剂量组(1 mg/L),氟中剂量组(5 mg/L)、氟高剂量组(25 mg/L)。经饮用水染毒3个月。计算脏器系数、测量骨组织氟浓度,用试剂盒测定血清中血清超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量。结果染毒过程中各组之间体重差异无统计学意义(P>0.05)。肝脏脏器系数从高到低依次为对照组、氟低剂量组、氟中剂量组和氟高剂量组(46.56±2.46)、(47.03±6.25)、(45.84±5.90)、(42.19±3.89),氟高剂量组与对照组相比显著下降,差异有统计学意义(P<0.05),其余各脏器系数变化无统计学意义(P>0.05)。中、高剂量(5.46±0.26)、(5.46±0.33)染氟组股骨脏器系数显著增加,与对照组(4.98±0.25)比较,差异有统计学意义(P<0.05)。染毒后骨组织中氟浓度显著升高,从低到高依次为对照组、低浓度组、中浓度组和高浓度组(711.37±263.83)、(998.75±254.87)、(1 195.21±159.73)、(1 957.00±273.19)μg/g,其中,中、高染氟组与对照组比较,差异有统计学意义(P<0.05)。氟高剂量组SOD活性(0.77±0.03)U/ml与对照组(0.86±0.07)U/ml比较显著降低(P﹤0.05);氟低剂量组、氟中剂量组、氟高剂量组(7.61±0.86)、(8.65±1.02)、(8.75±1.47)nmol/ml与对照组(4.36±0.36)nmol/ml比较MDA活性均升高,差异有统计学意义(P﹤0.05)。结论染毒后血清中SOD活力下降,各组MDA水平显著升高,提示存在氧化应激损伤。 Objective To investigate the oxidative damage induced by sub-chronic sodium fluoride in female mice. Methods Forty female mice were randomly divided into 4 groups (n = 10). In the control group, the low-dose fluoride group (1 mg / L), the middle-dose fluoride group (5 mg / L) and the high fluoride group (25 mg / L) After drinking water for 3 months. The organ coefficient was calculated and the fluoride concentration in bone tissue was measured. The activity of serum superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in serum were measured by kit. Results There was no significant difference in body weight between groups during exposure (P> 0.05). The coefficients of liver organ from high to low were as follows: control group, fluorine low dose group, middle fluoride dose group and high fluorine dose group (46.56 ± 2.46), (47.03 ± 6.25), (45.84 ± 5.90), (42.19 ± 3.89) (P <0.05). There was no significant difference in the other organ coefficients (P> 0.05). There was no significant difference between the high fluoride group and the control group (P> 0.05). The organ coefficient of femur in middle dose and high dose (5.46 ± 0.26) and (5.46 ± 0.33) group increased significantly compared with control group (4.98 ± 0.25), the difference was statistically significant (P <0.05). The concentration of fluoride in bone tissue increased significantly from low to high in the low concentration group, middle concentration group and high concentration group (711.37 ± 263.83), (998.75 ± 254.87), (1 195.21 ± 159.73) , (1 957.00 ± 273.19) μg / g respectively. There was a significant difference between the medium and high fluoride groups and the control group (P <0.05). (P <0.05). Compared with the control group (0.86 ± 0.07) U / ml, the activity of SOD in high fluoride group (0.77 ± 0.03) U / ml was significantly lower than that in the control group (P <0.05). Compared with the control group (4.36 ± 0.36) nmol / ml, MDA activity was significantly higher than that of the control group (± 0.86), (8.65 ± 1.02) and (8.75 ± 1.47) nmol / ml respectively. Conclusion The activity of SOD in serum decreased after treatment, and the level of MDA in each group was significantly increased, suggesting the existence of oxidative stress injury.