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目的 探讨日本血吸虫感染C57BL/6小鼠脾脏不同亚群调节性B细胞的含量.方法 使用日本血吸虫尾蚴经腹部感染C57BL/6小鼠,5周后分离感染组及正常对照组小鼠脾细胞,制备单个细胞悬液.使用细胞表面染色的方法,通过流式细胞术检测小鼠CD19+B细胞含量及表达免疫调节相关分子CD5、CD11b以及PD-1的B细胞亚群的含量.结果 C57BL/6小鼠感染日本血吸虫后,脾脏B细胞的百分比含量及数目明显增多,差异有统计学意义(P<0.01);CD5+和CD11b+B细胞的含量明显增加,差异有统计学意义(P0.05).结论 日本血吸虫感染能在C57BL/6小鼠体内诱导CD5+和CD11b+调节性B细胞产生.“,”Objective To explore the content of different subset of regulatory B cells in the spleen of Schistosoma japonicum infected C57BL/6 mouse. Methods C57BL/6 mice were percutaneously infected with Schistosoma japonicum(S. japonicum) cercariae. Five weeks later ,splenocytes were isolated ,and single cell solutions were made. The content of CD19 + B cells,and the expression of Immunomodulatory related molecule:CD5,CD11b,and PD-1 on B cells were investigated by the means of cell surface staining,and detected by Fluorescence activated cell sorting (FACs). Results After S. japonicum infection,the percentages and numbers of B cells in the spleen were increased significantly(P<0.01). The percentages of CD5+and CD11b+B cells were also increased(P0.05). Conclusion S. japonicum infection could up regulate CD5+ and CD11b+ regulatory B cells in the spleen of C57BL/6 mice.