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目的:讨论处理标本的温度对唐筛结果的影响。方法:应用时间分辨荧光免疫分析技术检测不同温度下分离的孕中期母血清中的生化指标,利用Life Cycle软件计算风险值,并进行统计学分析。结果:室温时AFP均值为0.94MOM,β-h CG均值为1.04MOM;37℃时AFP均值为0.95MOM;37℃时的β-h CG值明显高于室温(18℃~25℃)(t=0.45,P<0.001)。37℃处理标本时,唐筛高危检测阳性率高于室温(χ2=22.73,P<0.05)。结论:进行标本处理时,非抗凝静脉血静置的温度和时间对唐筛结果有很大影响。实验分析前的质量控制对于实验结果至关重要。
Objectives: To discuss the effect of the temperature of the specimen on the results of Tang sieve. Methods: Time-resolved fluorescence immunoassay was used to detect biochemical indicators of pregnant maternal serum separated at different temperatures. Life Cycle software was used to calculate the risk and the statistical analysis was performed. Results: The average AFP at room temperature was 0.94MOM, the average value of β-h CG was 1.04MOM. The mean AFP at 37 ℃ was 0.95MOM. The value of β-h CG at 37 ℃ was significantly higher than that at room temperature (18 ℃ -25 ℃) = 0.45, P <0.001). At 37 ℃, the positive rate of Tang sieve high-risk test was higher than that of room temperature (χ2 = 22.73, P <0.05). CONCLUSION: The temperature and time of non-anticoagulant venous blood have a great influence on the results of Tang sieve. Quality control before experimental analysis is crucial to the experimental results.