应用非同位素ＰＣＲ－ＳＳＣＰ－ＥＢ染色技术检测新鲜头颈部鳞癌组织中ｐ５３基因点突变。结果显示１３例标本中７例有异常电泳带，其中位于第５外显子者１例，位于第６，７，８外显子者各２例，说明头颈部鳞癌的发生与ｐ５３基因突变有密切关系。与传统的同位素ＰＣＲ－ＳＳＣＰ相比，非同位素ＰＣＲ－ＳＳＣＰ－ＥＢ染色技术是一种更简便、安全、快速、有效的检测基因点突变的方法，适用于大量标本基因点突变的筛选性检测。 Non-isotopic PCR-SSCP-EB staining technique was used to detect p53 gene point mutations in fresh head and neck squamous cell carcinoma. The results showed that 7 out of 13 specimens had abnormal electrophoresis bands, of which 1 was located in exon 5 and 2 exons were exon 6, 7, and 8 respectively, indicating the occurrence of head and neck squamous cell carcinoma and p53 gene. Mutations are closely related. Compared with conventional isotope PCR-SSCP, non-isotope PCR-SSCP-EB staining technology is a simpler, safer, faster, and more effective method for detecting gene point mutations, and is suitable for the screening detection of point mutations in a large number of specimens.