论文部分内容阅读
本文对实验制备的变链菌Ⅰ型ＪＢＰ（ｃ）和变链菌Ⅲ型６７１５（ｇ）含酶上清液和含酶提取液在外加引物后合成葡聚糖能力进行研究，发现ＪＢＰ（ｃ）含酶上清液合成水不溶性葡聚糖及６７１５（ｇ）含酶上清液合成水溶性和水不溶性葡聚糖均明显增加（Ｐ＜０．０５）。两种变链菌含酶提取液合成水溶性和水不溶性葡聚糖均无明显改变（Ｐ＞０．０５）。结果提示，ＪＢＰ（ｃ）含酶上清液中存在的ＧＴＦ－Ｉ和６７１５（ｇ）含酶上清液中存在的ＧＴＦ－Ｉ及ＧＴＦ－Ｓ可能为外源性引物依赖性ＧＴＦ，两种变链菌含酶提取液中存在的ＧＴＦ－Ｉ及ＧＴＦ－Ｓ可能为外源性引物非依赖性ＧＴＦ。可以推测，上清液中存在的外源性引物依赖性ＧＴＦ－Ｉ及其合成的ＩＧ在蔗糖依赖性粘附中可能起主导作用。
In this paper, the experimental preparation of mutans Streptococcus mutans type JBP (c) and Streptococcus mutans type III 6715 (g) containing enzyme supernatant and enzyme-containing extract with the addition of primers after glucan synthesis ability to study and found JBP (c ), The water-insoluble glucan synthesized from enzyme-containing supernatant and the water-soluble and water-insoluble glucan from 6715 (g) enzyme-containing supernatant increased significantly (P <0.05). There was no significant difference in the synthesis of water-soluble and water-insoluble dextran between the two Streptococcus mutans enzyme-containing extracts (P> 0.05). The results suggest that GTF-I and GTF-S present in the supernatant of GTF-I and 6715 (g) contained in JBP (c) enzyme-containing supernatant may be exogenous primer-dependent GTF, GTF-I and GTF-S present in the streptococcal enzyme-containing extract may be exogenous primer-independent GTF. Presumably, the exogenous primer-dependent GTF-I present in the supernatant and its synthetic IG may play a dominant role in sucrose-dependent adhesion.