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在正交设计对鱼腥草SRAP-PCR反应体系进行优化的基础上,分析鱼腥草居群的遗传多样性的结果表明:最佳的SRAP-PCR反应体系为每10μL溶液中含有0.2mmol·L-1dNTPs、20ng模板DNA、30ng·μL-1引物、0.5UTaq聚合酶和2mmol·L-1MgCl2;最佳的复性温度和循环次数为53℃和35次;从340个引物组合中筛选出条带清晰、多态性好的118个引物组合,并扩增出7582个谱带,多态性谱带有6590个,多态率为86.92%;在这些谱带中发现19条特异性的谱带,其中居群ZY42占31.58%;聚类分析结果显示鱼腥草居群存在非常丰富的遗传变异。
Based on the orthogonal design, the genetic diversity of Houttuynia cordata populations was analyzed based on the optimized SRAP-PCR reaction system. The optimal SRAP-PCR reaction system was 0.2 mmol · L -1 L-1dNTPs, 20ng template DNA, 30ng · μL-1 primer, 0.5UTaq polymerase and 2mmol·L-1MgCl2. The optimal annealing temperature and cycle number were 53 ℃ and 35 times. A total of 118 primer combinations with clear bands and good polymorphism were obtained and 7,582 bands were amplified. The number of polymorphic bands was 6590 with a polymorphism rate of 86.92%. Nineteen specific Among them, population ZY42 accounted for 31.58%. Cluster analysis showed that Houttuynia cordata populations were very rich in genetic variation.