组织原位分析鼻咽癌细胞核的DNA干系倍体及其异质性。收集42例鼻咽癌病例的归档蜡块,4μm、8μm连续组织学切片各一张。每个病例选取三个癌巢或肿瘤组织区域(共126个样本),使用TIGER细胞图像分析仪4μm组织切片测量鼻咽癌细胞核DNA的光密度等参数,8μm组织切片测量单个完整鼻咽癌细胞核的体积等参数,计算获得每个癌巢或肿瘤组织区域的以单个完整鼻咽癌细胞核的体积为单位的DNA含量(以体积积分光密度表述),以同一切片内正常鼻咽部上皮非基底细胞作为其内参照,计算其DNA干系倍体。结果显示:鼻咽癌细胞核的DNA干系倍体主要集中在1.76～3.00(DNA指数为0.88～1.50)之间,14个样本为DNA干系四倍体,仅2个样本为DNA干系亚二倍体;DNA干系倍体异质性率为66.67%。可得结论:(1)鼻咽癌DNA干系倍体主要在近二倍体与近四倍体之间;(2)鼻咽癌中存在DNA干系倍体异质性。 Tissue in situ analysis of DNA in the nucleus of nasopharyngeal carcinoma ploidy and its heterogeneity. Forty-two cases of nasopharyngeal carcinoma were collected and filmed with paraffin blocks, each of 4μm and 8μm serial histological sections. In each case, three cancer neoplasms or tumor tissue regions (126 samples in total) were selected. The optical density of nasopharyngeal carcinoma cell nuclear DNA was measured using TIGER 4 μm tissue section, and 8 μm tissue sections were used to measure single intact nasopharyngeal carcinoma cell nuclei Of the volume and other parameters calculated to obtain each cancer nests or tumor tissue area DNA content in single intact nasopharyngeal carcinoma cell nucleus volume (expressed as the volume integral optical density) to the same slice of normal nasopharyngeal epithelial non-basal Cells as its internal reference, calculate the DNA dry ploidy. The results showed that the DNA-based haploid of nasopharyngeal carcinoma cell nucleus mainly concentrated in the range of 1.76-3.00 (DNA index 0.88-1.50), 14 samples were tetraploid of DNA and only 2 samples were sub-diploid of DNA DNA haploid ploidy rate was 66.67%. Available conclusions: (1) nasopharyngeal cancer DNA ploidy mainly in the near diploid and nearly tetraploid; (2) there is DNA in the NPC pharyngeal heterogeneity.