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小麦籽粒中的多酚氧化酶活性是导致酶促褐变的主要因素。选育低多酚氧化酶活性的品种是改良面制食品外观品质的重要途径之一。本研究利用位于小麦2A和2D染色体上PPO基因分子标记PPO18和STS01,对扬麦158×淮麦18组合的300个F4代分离株系进行PCR扩增,说明不同带型与PPO活性的相关性。结果表明用PPO18扩增的300个分离株系中有59个扩增出876bp(2Aaa型)的目标片段,其活性均值为82.01;46个同时扩增出685bp和876bp的目标片段(2Aab型)其活性均值为163.41;其余195个扩增出685bp的目标片段(2Abb型)其活性均值为233.73。方差分析表明三者的PPO活性差异达到极显著水平,其中基因型为2Aaa型的PPO活性值明显低于2Abb型;PPO18在本试验群体中对PPO活性的决定系数为66.61%;用STS01扩增的300个株系中都扩增出了560bp的片段,群体间没有差异。文中说明了两对引物在该群体中的效应及其与PPO活性的关系。
Polyphenol oxidase activity in wheat grain is a major contributor to enzymatic browning. Breeding low polyphenol oxidase activity of the variety is one of the important ways to improve the quality of pasta food appearance. In this study, PPO18 and STS01, PPO gene markers located on chromosomes 2A and 2D of wheat, were used to amplify 300 F4 lines of combinations of Yangmai 158 × Huai-mai 18, indicating the correlation between different bands and PPO activity . The results showed that 59 out of 300 isolates amplified by PPO18 amplified 876bp (2Aaa type) of the target fragment, the average activity was 82.01; 46 simultaneous amplification of the target fragment of 685bp and 876bp (2Aab type) The average activity was 163.41. The remaining 195 amplified 685bp target fragments (2Abb type) with an average activity of 233.73. Analysis of variance showed that the PPO activity of the three groups reached a significant level. The PPO activity of 2Aaa genotype was significantly lower than that of 2Abb genotype. The coefficient of determination of PPO18 in this test population was 66.61% Of the 560 lines amplified in all of the 300 lines, no difference between the groups. The effect of two pairs of primers in this population and their relationship with PPO activity are described.