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目的:建立南五味子中核苷类成分的分析方法,对其核苷类成分进行分析评价,为南五味子资源的综合利用提供科学依据。方法:采用UPLC-TQ/MS技术分析测定9个产地、70批南五味子样品中16种核苷类成分的含量。使用ACQUITY UPLC BEH Amide色谱柱(100 mm×2.1 mm,1.7μm),以10 mmol·L-1乙酸胺+0.8%乙酸的水溶液(A)-0.05%乙酸的乙腈溶液(B)为流动相,梯度洗脱(0~6 min,10%A;6~8 min,10%A→40%A;8~9 min,40%A;9~11 min,40%A→50%A),流速0.4m L·min-1,柱温35℃。多反应监测(MRM)模式。结果:南五味子中含有多种核碱基、核苷和核苷酸类成分,所测成分中含量最高的是尿苷,含量最低的是2’-脱氧鸟苷。不同产地间核苷种类几无差异,不同产地间含量差异最大的成分是腺苷,各成分含量差异可能与地域有关。结论:建立了准确高效分析评价南五味子中核苷类成分的方法,研究结果可为南五味子功效物质基础的进一步研究和其资源开发利用提供科学依据。
OBJECTIVE: To establish a method for the analysis of the nucleosides in S. kansuensis, and to analyze and evaluate its nucleosides, so as to provide a scientific basis for the comprehensive utilization of S. kadsura resources. Methods: UPLC-TQ / MS was used to determine the content of 16 kinds of nucleoside components in nine samples from nine producing areas and 70 batches of Schisandra chinensis. The mobile phase consisted of 10 mmol·L-1 ammonium acetate + 0.8% acetic acid in water (A) and 0.05% acetic acid in acetonitrile (B) using an ACQUITY UPLC BEH Amide column (100 mm × 2.1 mm, Gradient elution (0-6 min, 10% A; 6-8 min, 10% A → 40% A; 8-9 min, 40% A; 9-11 min, 40% A → 50% A) 0.4m L · min-1, column temperature 35 ℃. Multiple Reaction Monitoring (MRM) mode. Results: Kadsura contains a variety of nucleobases, nucleosides and nucleotide components, the highest content of the measured components of uridine, the lowest content of 2’-deoxyguanosine. There is no difference in the types of nucleosides between different producing areas, the content of different origin is the largest difference between the composition of adenosine, the content of each component may be related to the region. Conclusion: The accurate and efficient method for the analysis and evaluation of the nucleosides in S. kansuensis was established. The results provide a scientific basis for the further study on the basis of Kadsura efficacy substance and its exploitation and utilization of resources.