用克山病病区低硒粮喂养大鼠,动态观察硒对心肌线粒体鱼藤酮不敏感的NADH—细胞色素C还原酶(RINCR)活性的影响。研究结果表明,病区粮喂养30d后心肌硒含量和线粒体谷胱甘肽过氧化物酶(GPX)活性明显下降,补硒可维持心肌硒水平和GPX活性接近常备饲料组大鼠水平或超过之。病区粮喂养30和60d时,心肌线粒体RINCR活性明显降低;至90d时有所恢复,但仍低于补硒和常备饲料组。补硒60和90d时,RINCR活性明显高于病区粮组,于90d时与常备饲料组已无明显差异。结果说明,克山病病区粮喂养大鼠心肌线粒体RINCR活性下降,补硒可部分恢复之,提示病区粮中除低硒外,还可能存在其它致病因素。 The rats fed with low selenium diet on Keshan disease were used to dynamically observe the effect of selenium on the mitochondrial rotenone insensitive NADH-cytochrome C reductase (RINCR) activity in myocardium. The results showed that myocardial selenium content and mitochondrial glutathione peroxidase (GPX) activity were significantly decreased 30 days after food-feeding in ward, selenium supplementation could maintain myocardial selenium level and GPX activity close to or exceed the level of normal rats . At 30 and 60 days, the mitochondrial RINCR activity of myocardial mitochondria decreased significantly, and restored to the level of 90 days, but still lower than that of selenium-enriched and normal feed group. At seventy and seventy selenium supplementation, the RINCR activity was significantly higher than that of the ward, and there was no significant difference between the two groups at 90 days. The results showed that the activity of mitochondrial RINCR decreased in myocardial-fed rats and the partial recovery of selenium could be recovered, suggesting that other disease-causing factors may exist in the diseased food besides selenium.