论文部分内容阅读
采用免疫组织化学ＡＢＣ法，对二乙基亚硝胺（ＤＥＮ）诱发大鼠肝癌发生过程中ｃ－ｆｏｓ蛋白在肝组织中的表达进行了系统观察。结果：正常大鼠肝细胞不表达ｃ－ｆｏｓ蛋白，诱癌第４周即可见少量肝细胞呈ｃ－ｆｏｓ蛋白阳性表达。随诱癌发展进程，肝细胞ｃ－ｆｏｓ蛋白表达逐渐增强，至诱癌中期（８～１２周），肝增生结节内部分肝细胞呈ｃ－ｆｏｓ蛋白阳性表达。诱癌晚期（１８周），癌结节内大部分肝癌细胞亦呈ｃ－ｆｏｓ蛋白阳性表达，ｃ－ｆｏｓ蛋白免疫反应阳性产物为胞核内棕褐色颗粒。提示肝细胞ｃ－ｆｏｓ蛋白的表达与肝癌的发生和发展具有相关性。
Immunohistochemical ABC method was used to observe the expression of c-fos protein in liver tissue during the development of rat liver cancer induced by diethylnitrosamine (DEN). RESULTS: Normal rat hepatocytes did not express c-fos protein. At the 4th week of induction, a small amount of hepatocytes showed c-fos protein positive expression. With the development of cancer induction, the expression of c-fos protein in hepatic cells gradually increased until the middle stage of cancer induction (8 to 12 weeks). Some hepatocytes in hepatic nodules showed c-fos protein positive expression. At the late stage of induction (18 weeks), most of the liver cancer cells in the tumor nodules also showed c-fos protein positive expression, and the positive product of c-fos protein immunoreactivity was nucleus tan particles. It is suggested that the expression of c-fos protein in liver cells is related to the occurrence and development of liver cancer.