作者应用高压液相色谱法同时测定多种维生素及多种维生素-多种矿物质片剂中的维生素B_1、B_2、B_6及烟酰胺。仪器为Waters 6000 A型溶剂泵;程序器;280nmUV检测器,自动进样器,μBondapak phenyl(10μm)30cm×3.9mm填充柱以及记录器。柱温30°,流动相为0.0025M己烷磺酸水溶液及甲醇,溶剂梯度0～80%甲醇,流速2ml/分。取B_1、B_6,烟酰胺标准品用0.1N盐酸配成0.5mg/ml;B_2标准品配成<0.1g/ Simultaneous Determination of Vitamin B_1, B_2, B_6 and Niacinamide in Multivitamin and Multivitamin - Multimineral Tablets by High Pressure Liquid Chromatography. The instrument was a Waters 6000 A solvent pump; a programmer; a 280 nm UV detector, autosampler, a μBondapak phenyl (10 μm) 30 cm × 3.9 mm packed column and recorder. The column temperature was 30 °, the mobile phase was 0.0025M hexanesulfonic acid aqueous solution and methanol, the solvent gradient was 0 ~ 80% methanol, the flow rate was 2ml / min. Take B_1, B_6, nicotinamide standard 0.1N hydrochloric acid dubbed 0.5mg / ml; B_2 standard dubbed <0.1g /